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Westernblot analysis using anti - human fibulin 5 monoclonal antibody agaist truncated Fibulin 5 recombinant protein and Hela lysate.
Immunohistochemical analysis of paraffin - embedded human stomach carcinoma (top) and human breast carcinoma (bottom) showing cytoplasmic localization using Fibulin 5 antibody with DAB staining.
Westernblot analysis using anti - human fibulin 5 monoclonal antibody agaist truncated Fibulin 5 recombinant protein and Hela lysate.

Fibulin5 Antibody [3F10A5 / 3F8A12]

CATALOG NUMBER: 32-155

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant Fibulin 5 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2000
IHC(P):1:500 - 1:2000
IHC(F):1:500 - 1:2000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Fibulin 5 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FBLN5
Additional Names:
DANCE, UNQ184/PRO210, Developmental arteries and neural crest EGF-like protein, FIBL-5, EVEC, UP50, ADCL2, ARMD3, DANCE, ARCL1A
Protein Accession Number:
Q9UBX5
Protein Gi Number:
12643876
Ncbi Gene Id Number:
10516
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fibulin 5(FBLN5), with 448-amino acid protein (about 50 kDa), is a recently discovered multifunctional extracellular matrix protein that mediates endothelial cell adhesion through integrin ligation, regulates cell growth and motility in a context-specific manner, and prevents elastinopathy in vivo. Fibulin-5 is abundantly expressed in great vessels and cardiac valves during embryogenesis, and in many adult tissues including the aorta, lung, uterus and skin, all of which contain abundant elastic fibres. Decreased fibulin-5 may contribute to the pathogenesis of aortic dissection by impairing elastic fiber assembly. Fibulin-5 is also a good marker of skin ageing and that the earlier loss of fibulin-5 may involve age-dependent changes in other elastic fibre components.
Background Reference 1:
Wen Wang , Scott A. LeMaire, Li Chen. Surgery. 2005 Aug;138(2):352-9
Background Reference 2:
K. Kadoya, T. Sasaki, G. Kostka. Br J Dermatol. 2005 Sep;153(3):607-123.
Background Reference 3:
Michael J. Lee, Nakshatra K. Roy, Jon E. Mogford. J Am Coll Surg. 2004 Sep;199(3):403-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-155

List Size:
0.1 mL

List Price:

$495.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

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