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IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1561). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Filaggrin Antibody (clone FLG/1561). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1561). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Filaggrin Antibody [FLG/1561]

CATALOG NUMBER: 33-694

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 min at RT
Optimal dilution of the Filaggrin antibody should be determined by the researcher.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
FLI1
Additional Names:
FLI1 Antibody : ATOD2, Filaggrin
Ncbi Gene Id Number:
2312
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-694

List Size:
100 ug

List Price:

$626.00

Datasheet
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