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43-533 (0.1ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-533 (0.1ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

filamin A Antibody

CATALOG NUMBER: 43-533

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DIRDAPQDFHPDRVK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 250kDa band observed in Human Uterus lysates (calculated MW of 281kDa according to NP_001447.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001447.2; NP_001104026.1).
Predicted Molecular Weight:
Approx 250kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
filamin A
Additional Names:
FLNA, filamin A, alpha, ABP-280, ABPX, CVD1, FLN, FLN-A, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2, XLVD, XMVD, actin binding protein 280, alpha-filamin, endothelial actin-binding protein, filamin-1, filamin-A, non-muscle filamin
Protein Accession Number:
NP_001447.2,NP_001104026.1
Protein Gi Number:
116241365
Ncbi Gene Id Number:
2316
Background Reference 1:
Guiet R, Vérollet C, Lamsoul I, Cougoule C, Poincloux R, Labrousse A, Calderwood DA, Glogauer M, Lutz PG, Maridonneau-Parini I.Macrophage mesenchymal migration requires podosome stabilization by filamin A.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-533

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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