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45-596 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-596 (5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-596 (5ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-596 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FIR Antibody

CATALOG NUMBER: 45-596

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-YDQERFDNSDLSA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human lysates of HEK293 (calculated MW of 59.9kDa according to NP_510965.1 and 58.2 according to NP_055096.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Skin. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1)
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PUF60
Additional Names:
PUF60, SIAHBP1, FIR, fuse-binding protein-interacting repressor, RoBPI, siah binding protein 1, poly-U binding splicing factor PUF60, Ro ribonucleoprotein-binding protein 1, pyrimidine tract binding splicing factor, poly-U binding splicing factor 60KDa, FLJ31379, FBP interacting repressor, Ro ribonucleoprotein binding protein 1
Protein Accession Number:
NP_510965.1,NP_055096.2,NP_001129505.1
Protein Gi Number:
17978512
Ncbi Gene Id Number:
22827
Background Reference 1:
Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D. The FBP interacting repressor targets TFIIH to inhibit activated transcription. Mol Cell. 2000 Feb;5(2):331-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-596

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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