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Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.
Immunocytochemistry of FKBP12 in A431 cells with FKBP12 antibody at 5 μg/mL.
Immunofluorescence of FKBP12 in A431 cells with FKBP12 antibody at 20 μg/mL.
Western blot analysis of FKBP12 in A431 cell lysate with FKBP12 antibody at 1 μg/ml.
Trial Size

FKBP12 Antibody

CATALOG NUMBER: 7789

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rabbit: (100%)
Immunogen:
FKBP12 antibody was raised against an 18 amino acid peptide near the center of human FKBP12.
The immunogen is located within the first 50 amino acids of FKBP12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FKBP12 antibody can be used for detection of FKBP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
FKBP12 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 17-002 - A-431 Cell Slide
Predicted Molecular Weight:
Predicted: 12 kDa
Observed: 12 kDa
Purification:
FKBP12 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FKBP12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FKBP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
FKBP1A
Additional Names:
FKBP12 Antibody: FKBP1, PKC12, PKCI2, FKBP12, PPIASE, FKBP-12, FKBP-1A, FKBP1, Peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, PPIase FKBP1A
Protein Accession Number:
NP_463460
Protein Gi Number:
17149836
Ncbi Gene Id Number:
2280
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins in function (1). FKBP12, also called FKBP1, was originally identified as a target of FK506 and rapamycin activity and catalyzes the transition between cis and transproline residues critical for proper folding of proteins (2). FKBP12 is an abundant, evolutionarily conserved cytoplasmic protein that is an important regulator of diverse array of cellular processes including T cell activation, entry into the cell cycle and intracellular calcium release (3-5).
Background Reference 1:
Siekierka JJ, Hung SH, Poe M, et al. A cytosolic binding protein for the immunosuppressant FK506 has peptidyl-prolyl isomerase activity but is distinct from cyclophilin. Nature 1989; 341:755-7.
Background Reference 2:
Michnick SW, Rosen MK, Wandless TJ, et al. Solution structure of FKBP, a rotamase enzyme and receptor for FK506 and rapamycin. Science 1991; 252:836-9.
Background Reference 3:
Wang T, Donahoe PK, and Zervos AS. Specific interaction of type I receptors of the TGF-beta family with the immunophilin FKBP-12. Science 1994; 265:674-6.
Background Reference 4:
Wen H, Kang S, Song Y, et al. Characterization of the binding sites for the interactions between FKBP12 and intracellular calcium release channels. Arch. Biochem. Biophys. 2012; 517:37-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7789

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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