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46-782 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-782 (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-782 (3.75ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-782 (0.01ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FKBP4 Antibody

CATALOG NUMBER: 46-782

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-DHPTDTEMKEEQKSN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 51.8kDa according to NP_002005.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney and Brain (Cerebellum). Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1309 - Human Placenta Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FKBP4
Additional Names:
FKBP4, FKBP52, FK506 binding protein 4, 59kDa, FKBP59, HBI, Hsp56, PPIase, p52, FK506 binding protein 4 (59kD), FK506-binding protein 4, FK506-binding protein 4 (59kD), HSP binding immunophilin, T-cell FK506-binding protein, 59kD, p59 protein, peptidylprolyl cis-trans isomerase, rotamase
Protein Accession Number:
NP_002005.1
Protein Gi Number:
4503729
Ncbi Gene Id Number:
2288
Background Reference 1:
Ruan B, Pong K, Jow F, Bowlby M, Crozier RA, Liu D, Liang S, Chen Y, Mercado ML, Feng X, Bennett F, von Schack D, McDonald L, Zaleska MM, Wood A, Reinhart PH, Magolda RL, Skotnicki J, Pangalos MN, Koehn FE, Carter GT, Abou-Gharbia M, Graziani EI. Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities. Proc. Natl. Acad. Sci. U.S.A. 2008 Jan 105 (1): 33-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-782

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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