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Western blot analysis of extracts of various cell lines, using FKBP8 antibody (22-685) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of A549 cells using FKBP8 antibody (22-685). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FKBP8 antibody (22-685). Western blot was performed from the immunoprecipitate using FKBP8 antibody (22-685) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using FKBP8 antibody (22-685) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

FKBP8 Antibody

CATALOG NUMBER: 22-685

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human FKBP8 (NP_036313.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
IP: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
THP-1
Positive Control 3:
BT-474
Positive Control 4:
HeLa
Positive Control 5:
HL-60
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 55kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FKBP8
Additional Names:
FKBP8, FKBP38, FKBPr38
Ncbi Gene Id Number:
23770
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. Unlike the other members of the family, this encoded protein does not seem to have PPIase/rotamase activity. It may have a role in neurons associated with memory function.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-685

List Size:
100 uL

List Price:

$436.00

Datasheet
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