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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).
<b>Top Image:</b> Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using FKHR (Phospho-Ser256).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using FKHR (Phospho-Ser256).
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using FKHR (Phospho-Ser256).

FKHR (phospho Ser256) Antibody

CATALOG NUMBER: 79-154

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FKHR (Phospho-Ser256) antibody was raised against a peptide sequence around phosphorylation site of serine 256 (A-A-S (p) -M-D) derived from Human FKHR.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of FKHR only when phosphorylated at serine 256.
Predicted Molecular Weight:
78 - 82 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
FOXO1
Additional Names:
FKH1, FKHR, FOXO1A, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, FOXO1
Protein Accession Number:
NP_002006.2
Protein Gi Number:
9257222
Ncbi Gene Id Number:
2308
Background:
Transcription factor.
Background Reference 1:
Gan L, et al. (2005) J Neurochem; 93 (5): 1209-19. Smith WW, et al. (2005) J Cell Biol; 169 (2): 331-9. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Horn S, et al. (2004) Leukemia; 18 (11): 1839-49.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-154

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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