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Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum starvation using FKHR (Phospho-Ser319).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR (Phospho-Ser319).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using FKHR (Phospho-Ser319).
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum starvation using FKHR (Phospho-Ser319).

FKHR (phospho Ser319) Antibody

CATALOG NUMBER: 79-167

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
FKHR (Phospho-Ser319) antibody was raised against a peptide sequence around phosphorylation site of serine 319 (T-S-S (p) -N-A) derived from Human FKHR/FOXO1A.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of FKHR only when phosphorylated at serine 319.
Predicted Molecular Weight:
78 - 82 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
FOXO1
Additional Names:
FKH1, FKHR, FOXO1A, MEK2, MKK2, PRKMK2, FOXO1
Protein Accession Number:
NP_002006.2
Protein Gi Number:
9257222
Ncbi Gene Id Number:
2308
Background:
FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14
Background Reference 1:
Rena G, et al. (2002) EMBO J 21 (9): 2263-2271. Woods YL, et al. (2001) Biochem J355 (Pt 3): 597-607. Rena G, et al. (2001) Biochem J 354 (Pt 3): 605-612.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-167

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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