Flagellin Recombinant Protein

CATALOG NUMBER: 90-279

Specifications
source-species:
Salmonella typhimurium strain 14028
Species:
Human, Mouse
source-species:
Salmonella typhimurium strain 14028
Application Note:
This recombinant proteins is for research use only.
Biological Activity:
Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice.TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1ug /mouse.
Purity:
>90% (SDS-PAGE).

Endotoxin level is less than 0.1EU/ μg purified protein (LAL test; Lonza).
physical-state:
Lyophilized
Buffer:
Contains PBS. Reconstitute with 100 μl sterile water.
Concentration:
1mg/ml after reconstitution.
Storage Conditions:
Stable for at least 6 months after receipt when stored at -20°C.
Ncbi Official Symbol:
fliC
Additional Names:
FliC
Protein Accession Number:
P06179
Protein Gi Number:
20141325
Ncbi Gene Id Number:
1253480
Background:
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

90-279

List Size:
100 ug

List Price:

$423.00

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