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Western blot analysis of FLI1 antibody in mouse lung tissue lysates(35ug/lane)
Confocal immunofluorescent analysis of FLI1 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Western blot analysis of FLI1 antibody in mouse lung tissue lysates(35ug/lane)

FLI1 Antibody

CATALOG NUMBER: 62-290

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Drosophila, Zebrafish, Rat, Bovine, Xenopus
Immunogen:
This FLI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-328 amino acids from the Central region of human FLI1.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FLI1
Additional Names:
Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
Protein Accession Number:
Q01543
Protein Gi Number:
399496
Ncbi Gene Id Number:
2313
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FLI1 is a sequence-specific transcriptional activator. It recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Background Reference 1:
Embree,L.J., Cancer Res. 69 (10), 4363-4371 (2009)
Background Reference 2:
Torlakovic,E.E., Histol. Histopathol. 23 (11), 1309-1314 (2008)
Background Reference 3:
Cironi,L., PLoS ONE(7), E2634 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-290

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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