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Western blot analysis of extracts of various cell lines, using FLII antibody (15-954) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5S.
Immunohistochemistry of paraffin-embedded rat heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FLII antibody (15-954) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FLII antibody (15-954) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using FLII antibody (15-954) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5S.

FLII Antibody

CATALOG NUMBER: 15-954

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human FLII (NP_002009.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
293T
Positive Control 3:
HeLa
Positive Control 4:
Jurkat
Predicted Molecular Weight:
Observed: 160kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FLII
Additional Names:
FLI, FLIL, Fli1, protein flightless-1 homolog, flightless I actin binding protein, flightless I homolog
Ncbi Gene Id Number:
2314
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-954

List Size:
100 uL

List Price:

$436.00

Datasheet
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