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Antibody used in IHC on Human kidney.
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Antibody used in IHC on Human kidney.

FLJ11730 Antibody

CATALOG NUMBER: 27-835

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FLJ11730.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
FLJ11730 antibody can be used for detection of FLJ11730 by ELISA at 1:312500. FLJ11730 antibody can be used for detection of FLJ11730 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
23 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store FLJ11730 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
MEAF6
Additional Names:
FLJ11730, EAF6, CENP-28, C1orf149, NY-SAR-91
Protein Accession Number:
NP_073593
Protein Gi Number:
40255020
Ncbi Gene Id Number:
64769
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The screening of cDNA expression libraries from human tumors with serum antibody (SEREX) has proven to be a powerful method for identifying the repertoire of tumor antigens recognized by the immune system of cancer patients, referred to as the cancer immunome. In this regard, cancer/testis (CT) antigens are of particular interest because of their immunogenicity and restricted expression patterns. Synoivial sarcomas are striking with regard to CT antigen expression, however, highly expressed in sarcoma, CT antigens do not induce frequent humoral immune responses in sarcoma patients. Sera from two patients were used to immunoscreen cDNA libraries from two synovial sarcoma cell lines and normal testis, resulting in the identification of 113 distinct antigens. Sarcoma antigen NY-SAR-91 is one of them.
Background Reference 1:
Lee, S.Y., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (5), 2651-2656.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-835

List Size:
100 ul

List Price:

$519.00

Datasheet
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