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Western blot analysis of extracts of various cell lines, using FLNB antibody (18-661) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody (18-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using FLNB antibody (18-661) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody (18-661) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HUVEC cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using FLNB antibody (18-661) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using FLNB antibody (18-661) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 10s.

FLNB Antibody

CATALOG NUMBER: 18-661

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1686-1785 of human FLNB (NP_001157789.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:100
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
A-431
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse uterus
Predicted Molecular Weight:
Observed: 298kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FLNB
Additional Names:
FLNB, filamin B, beta (actin binding protein 278), ABP-278, AOI, DKFZp686A1668, DKFZp686O033, FH1, FLN1L, LRS1, SCT, TABP, TAP, beta filamin, filamin 1 (actin-binding protein-280)-like, beta (actin-binding protein-278), FLN3
Ncbi Gene Id Number:
2317
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-661

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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