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Western blot analysis of extracts of various cell lines, using FLNC antibody (14-747) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using FLNC antibody (14-747) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using FLNC antibody (14-747) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

FLNC Antibody

CATALOG NUMBER: 14-747

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 2160-2340 of human FLNC (NP_001449.3).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
LO2
Positive Control 3:
NIH/3T3
Predicted Molecular Weight:
Observed: 302kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FLNC
Additional Names:
ABP-28ABP280A, ABPA, ABPL, CMH26, FLN2, MFM5, MPD4, RCM5, filamin-C, ABP-280-like protein, ABP-L, gamma filamin, actin binding protein 28filamin C, gamma, filamin-2, gamma filamin
Ncbi Gene Id Number:
2318
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of three related filamin genes, specifically gamma filamin. These filamin proteins crosslink actin filaments into orthogonal networks in cortical cytoplasm and participate in the anchoring of membrane proteins for the actin cytoskeleton. Three functional domains exist in filamin: an N-terminal filamentous actin-binding domain, a C-terminal self-association domain, and a membrane glycoprotein-binding domain. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-747

List Size:
100 uL

List Price:

$436.00

Datasheet
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