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Western blot analysis in HepG2 cell line lysates (35ug/lane).
Western blot analysis in HepG2 cell line lysates (35ug/lane).

FLOT1 Antibody

CATALOG NUMBER: 60-189

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Immunogen:
This FLOT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the N-terminal region of human FLOT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FLOT1
Additional Names:
Flotillin-1, FLOT1
Protein Accession Number:
O75955
Ncbi Gene Id Number:
10211
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Caveolae are small domains on the inner cell membraneinvolved in vesicular trafficking and signal transduction. FLOT1encodes a caveolae-associated, integral membrane protein. Thefunction of flotillin 1 has not been determined. [provided byRefSeq].
Background Reference 1:
Lee, B.Y., et al. Mol. Cell Proteomics 9(1):32-53(2010)
Background Reference 2:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) :
Background Reference 3:
Saalik, P., et al. Bioconjug. Chem. 20(5):877-887(2009)
Background Reference 4:
Rajendran, L., et al. PLoS ONE(12), E8290 (2009) :

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-189

List Size:
400 ul

List Price:

$495.00

Datasheet
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