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45-051 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-051 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-051 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Flotillin 1 Antibody

CATALOG NUMBER: 45-051

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SISQVNHKPLRTA
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell lines A431, Jurkat and K562 (calculated MW of 47.4kDa according to NP_005794.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
Positive Control 1:
H460 Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FLOT1
Additional Names:
FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2
Protein Accession Number:
NP_005794.1,NP_001305804.1
Protein Gi Number:
5031699
Ncbi Gene Id Number:
10211
Background Reference 1:
Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem. 1997 May 23;272(21):13793-802.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-051

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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