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Western blot analysis of FLT3 in 3T3 cell lysate with FLT3 antibody at (A) 1 and (B)2 μg/mL.
Immunofluorescence of FLT3 in human kidney tissue with FLT3 antibody at 20 μg/mL.
Western blot analysis of FLT3 in 3T3 cell lysate with FLT3 antibody at (A) 1 and (B)2 μg/mL.
Trial Size

FLT3 Antibody

CATALOG NUMBER: 7027

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3.
The immunogen is located within amino acids 340 - 390 of FLT3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
FLT3 antibody can be used for detection of FLT3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
109 kDa
Purification:
FLT3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FLT3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FLT3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FLT3
Additional Names:
FLT3 Antibody: FLK2, STK1, CD135, FLK-2, FLK2, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor
Protein Accession Number:
NP_004110
Protein Gi Number:
121114304
Ncbi Gene Id Number:
2322
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FLT3 Antibody: FLT3 is a member of the class III receptor tyrosine kinase family and its internal tandem duplication (ITD) is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. It is a membrane bound glycosylated protein that is expressed primarily in early hematopoietic progenitor cells. Upon binding its ligand FL, FLT3 activates multiple signaling pathways including the PI3K/Akt and Ras/MEK/MAPK pathways. In cells expressing FLT3-ITD, these pathways are constitutively on, resulting in abnormal cell growth and anti-apoptotic activity.
Background Reference 1:
Rosnet O, Schiff C, Pebusque MJ, et al. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993; 82:1110-9
Background Reference 2:
Yokoa S, Kiyoi H, Nakao M, et al. Internal tandem duplication of the FLT3 gene is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. Leukemia 1997; 11:1605-9.
Background Reference 3:
Takahashi S. Downstream molecular pathways of FLT3 in the pathogenesis of acute myeloid leukemia: biology and therapeutic implications. J. Hematol. and Oncol. 2011; 4:13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7027

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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