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Western blot analysis of extracts of mouse liver, using FLT4 antibody (19-753) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human colon using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using FLT4 antibody (19-753) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using FLT4 antibody (19-753) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse liver, using FLT4 antibody (19-753) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

FLT4 Antibody

CATALOG NUMBER: 19-753

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1174-1363 of human FLT4 (NP_891555.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse liver
Predicted Molecular Weight:
Observed: 153kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FLT4
Additional Names:
FLT4, FLT41, Fms-related tyrosine kinase 4, Fms-like tyrosine kinase 4, LMPH1A, PCL, Soluble VEGFR3 variant 1, Vegf-r3, VEGFR3, Soluble VEGFR3 variant 2, Soluble VEGFR3 variant 3, FLT-4, Quek2, VEGF Receptor 3, VEGFR-3
Ncbi Gene Id Number:
2324
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-753

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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