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Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with FMOD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of FMOD Antibody in mouse cerebellum tissue lysates (35ug/lane)

FMOD Antibody

CATALOG NUMBER: 63-852

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This FMOD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from the C-terminal region of human FMOD.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FMOD
Additional Names:
Fibromodulin, FM, Collagen-binding 59 kDa protein, Keratan sulfate proteoglycan fibromodulin, KSPG fibromodulin, FMOD, FM, SLRR2E
Protein Accession Number:
Q06828
Protein Gi Number:
223590208
Ncbi Gene Id Number:
2331
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix.
Background Reference 1:
Ehret,G.B., et.al, Eur. J. Hum. Genet. 17 (12), 1650-1657 (2009)
Background Reference 2:
Tillgren,V., et.al, J. Biol. Chem. 284 (42), 28543-28553 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-852

List Size:
400 ul

List Price:

$495.00

Datasheet
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