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Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody at 1:1000.
FOLR1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody at 1:1000.

FOLR1 Antibody

CATALOG NUMBER: 55-347

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FOLR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-68 amino acids from the N-terminal region of human FOLR1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOLR1
Additional Names:
Folate receptor alpha, FR-alpha, Adult folate-binding protein, FBP, Folate receptor 1, Folate receptor, adult, KB cells FBP, Ovarian tumor-associated antigen MOv18, FOLR1, FOLR
Protein Accession Number:
P15328
Protein Gi Number:
544337
Ncbi Gene Id Number:
2348
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the folatereceptor family. Members of this gene family bind folic acid andits reduced derivatives, and transport 5-methyltetrahydrofolateinto cells. This gene product is a secreted protein that eitheranchors to membranes via a glycosyl-phosphatidylinositol linkage orexists in a soluble form. Mutations in this gene have beenassociated with neurodegeneration due to cerebral folate transportdeficiency. Due to the presence of two promoters, multipletranscription start sites, and alternative splicing, multipletranscript variants encoding the same protein have been found forthis gene.
Background Reference 1:
Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
O'Byrne, M.R., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(8):689-694(2010)
Background Reference 4:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-347

List Size:
400 ul

List Price:

$495.00

Datasheet
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