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Western blot analysis in U251 cell line lysates (35ug/lane).
Fluorescent confocal image of A549 cell stained with FOSL2 Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOSL2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOSL2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in U251 cell line lysates (35ug/lane).

FOSL2 Antibody

CATALOG NUMBER: 58-520

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FOSL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-248 amino acids from the C-terminal region of human FOSL2.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOSL2
Additional Names:
Fos-related antigen 2, FRA-2, FOSL2, FRA2
Protein Accession Number:
P15408
Protein Gi Number:
120487
Ncbi Gene Id Number:
2355
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Fos gene family consists of 4 members: FOS, FOSB,FOSL1, and FOSL2. These genes encode leucine zipper proteins thatcan dimerize with proteins of the JUN family, thereby forming thetranscription factor complex AP-1. As such, the FOS proteins havebeen implicated as regulators of cell proliferation,differentiation, and transformation.
Background Reference 1:
Bozec, A., et al. J. Cell Biol. 190(6):1093-1106(2010)
Background Reference 2:
Bolat, I., et al. Folia Histochem. Cytobiol. 48(2):197-201(2010)
Background Reference 3:
Reich, N., et al. Arthritis Rheum. 62(1):280-290(2010)
Background Reference 4:
Maurer, B., et al. Circulation 120(23):2367-2376(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-520

List Size:
400 ul

List Price:

$495.00

Datasheet
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