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45-602 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
45-602 (1ug/ml) staining of HepG2 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

FOXB1 Antibody

CATALOG NUMBER: 45-602

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-TSPASALHSVAVH
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 35.0kDa according to NP_036314.2. Recommended concentration: 1-3ug/ml.Immunocytochemsitry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
Immunofluorescence: This antibody has been successfully used in IF for mouse brain at embryonic day 13.5.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXB1
Additional Names:
FOXB1, forkhead box B1, FKH5, HFKH-5
Protein Accession Number:
NP_036314.2
Protein Gi Number:
105554437
Ncbi Gene Id Number:
27023
Background Reference 1:
Kloetzli JM, Fontaine-Glover IA, Brown ER, Kuo M, Labosky PA. The winged helix gene, Foxb1, controls development of mammary glands and regions of the CNS that regulate the milk-ejection reflex. Genesis. 2001 Feb;29(2):60-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-602

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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