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45-605 staining (2µg/ml) of nuclear HEK293 (A) and (1ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
45-605 (2ug/ml) staining of paraffin embedded Human Breast
45-605 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
45-605 staining (2µg/ml) of nuclear HEK293 (A) and (1ug/ml) negative control Human Pancreas (B) lysate [RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

FOXC2 Antibody

CATALOG NUMBER: 45-605

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: RHAAPYSYDCTKY
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in lysates of cell lines K562 and HeLa, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 38.8kDa according to Human NP_075555.1). Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature.
Positive Control 1:
Cat. No. 1323 - Human Breast Tumor Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXC2
Additional Names:
FOXC2, forkhead box C2 (MFH-1, mesenchyme forkhead 1), MFH1, MFH-1, FKHL14, MFH-1,mesenchyme forkhead 1, forkhead (Drosophila)-like 14, forkhead, Drosophila, homolog-like 14, forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1), LD, forkhead box C2, forkhead, Drosophila, homolog-like 14
Protein Accession Number:
NP_005242.1
Protein Gi Number:
4885237
Ncbi Gene Id Number:
2303
Background Reference 1:
Brice G, Mansour S, Bell R, Collin JR, Child AH, Brady AF, Sarfarazi M, Burnand KG, Jeffery S, Mortimer P, Murday VA. Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24. J Med Genet. 2002 Jul;39(7):478-83.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-605

List Size:
0.1 mg

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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