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Western blot analysis in A2058 cell line lysates (15ug/lane).
immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the for immunohistochemistry.
Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A2058 cell line lysates (15ug/lane).

FOXC2 Antibody

CATALOG NUMBER: 62-034

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FOXC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the Central region of human FOXC2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXC2
Additional Names:
Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH-1 protein, Transcription factor FKH-14, FOXC2, FKHL14, MFH1
Protein Accession Number:
Q99958
Protein Gi Number:
3024149
Ncbi Gene Id Number:
2303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXC2 belongs to the forkhead family of transcriptionfactors which is characterized by a distinct DNA-binding forkheaddomain. The specific function of this gene has not yet beendetermined; however, it may play a role in the development ofmesenchymal tissues.
Background Reference 1:
van Dongen, M.J., et al. J. Mol. Biol. 296(2):351-359(2000)
Background Reference 2:
Mangion, J., et al. Am. J. Hum. Genet. 65(2):427-432(1999)
Background Reference 3:
Miura, N., et al. Genomics 41(3):489-492(1997)
Background Reference 4:
Kaestner, K.H., et al. Development 122(6):1751-1758(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-034

List Size:
400 ul

List Price:

$495.00

Datasheet
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