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FOXF1 Antibody

CATALOG NUMBER: 43-447

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-QNSHNAPAELQGIPR
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of cell line MRC5 PD19 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.1kDa according to NP_001442.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXF1
Additional Names:
FOXF1, forkhead box F1, ACDMPV, FKHL5, FREAC1, FREAC-1, Forkhead, drosophila, homolog-like 5, forkhead box protein F1, forkhead-related activator 1, forkhead-related protein FKHL5, forkhead-related transcription factor 1
Protein Accession Number:
NP_001442.2
Protein Gi Number:
110735445
Ncbi Gene Id Number:
2294
Background Reference 1:
Saito RA, Micke P, Paulsson J, Augsten M, Peña C, Jönsson P, Botling J, Edlund K, Johansson L, Carlsson P, Jirström K, Miyazono K, Ostman A.Forkhead box F1 regulates tumor-promoting properties of cancer-associated fibroblasts in lung cancer.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-447

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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