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45-053 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-053 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FOXG1 Antibody

CATALOG NUMBER: 45-053

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-HQNQGSSSNPLIH
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48 kDa band observed in Human Brain lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B) and 50.5kDa according to CAA55038.1 (FOXG1A)). Recommended concentration: 0.3-1.0ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 70kDa. This band was successfully blocked by incubation with the immunising peptide. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXG1
Additional Names:
forkhead box G1, BF1, BF2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF-2, HBF-3, HBF-G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN, OTTHUMP00000178764, brain factor 2, forkhead box G1A, forkhead box G1B, forkhead box G1C, forkhead, drosophila, homolog-like 2, forkhead-like 1, forkhead-like 2, forkhead-like 3, forkhead-like 4, oncogene QIN
Protein Accession Number:
NP_005240.3
Protein Gi Number:
967048
Ncbi Gene Id Number:
2290
Background Reference 1:
Wiese S, Murphy DB, Schlung A, Burfeind P, Schmundt D, Schnulle V, Mattei MG, Thies U. The genes for human brain factor 1 and 2, members of the fork head gene family, are clustered on chromosome 14q. Biochim Biophys Acta. 1995 Jun 9;1262(2-3):105-12.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-053

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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