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Western Blot at 1:500 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:500 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.

FOXK1 Antibody

CATALOG NUMBER: 64-162

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This FOXK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-710 amino acids from the C-terminal region of human FOXK1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:500
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXK1
Additional Names:
Forkhead box protein K1, Myocyte nuclear factor, MNF, FOXK1 {ECO:0000303, PubMed:15202027, ECO:0000303, PubMed:15289879}
Protein Accession Number:
P85037
Protein Gi Number:
118572324
Ncbi Gene Id Number:
221937
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXK1 is a transcriptional activator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. It plays a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.
Background Reference 1:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 2:
Tsai, K.L., et al. J. Biol. Chem. 281(25):17400-17409(2006)
Background Reference 3:
Huang, J.T., et al. Int. J. Oncol. 25(3):751-757(2004)
Background Reference 4:
Katoh, M., et al. Int. J. Mol. Med. 14(1):127-132(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-162

List Size:
400 ul

List Price:

$495.00

Datasheet
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