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45-617 (2µg/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35µg protein in RIPA buffer). Detected by chemiluminescence.
45-617 (2ug/ml) staining of paraffin embedded Human Ovary. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-617 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-617 (2µg/ml) staining of K562 cell lysate (A) + peptide (B) and HeLa cell lysate (C) + peptide (D) 35µg protein in RIPA buffer). Detected by chemiluminescence.

FOXL2 Antibody

CATALOG NUMBER: 45-617

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: C-DSKTGALHSRLDL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1316 - Human Ovary Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXL2
Additional Names:
FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3
Protein Accession Number:
NP_075555.1
Protein Gi Number:
12751477
Ncbi Gene Id Number:
668
Background Reference 1:
Crisponi L, Deiana M, Loi A, Chiappe F, Uda M, Amati P, Bisceglia L, Zelante L, Nagaraja R, Porcu S, Ristaldi MS, Marzella R, Rocchi M, Nicolino M, Lienhardt-Roussie A, Nivelon A, Verloes A, Schlessinger D, Gasparini P, Bonneau D, Cao A, Pilia G. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet. 2001 Feb;27(2):159-66.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-617

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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