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Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

FOXN1 Antibody

CATALOG NUMBER: 56-203

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This FOXN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-378 amino acids from the Central region of human FOXN1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:2000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXN1
Additional Names:
Forkhead box protein N1, Winged-helix transcription factor nude, FOXN1, RONU, WHN
Protein Accession Number:
O15353
Ncbi Gene Id Number:
8456
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mutations in the winged-helix transcription factor gene atthe nude locus in mice and rats produce the pleiotropic phenotypeof hairlessness and athymia, resulting in a severely compromisedimmune system. This gene is orthologous to the mouse and rat genesand encodes a similar DNA-binding transcription factor that isthought to regulate keratin gene expression. A mutation in thisgene has been correlated with T-cell immunodeficiency, the skindisorder congenital alopecia, and nail dystrophy. Alternativesplicing in the 5' UTR of this gene has been observed. [provided byRefSeq].
Background Reference 1:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
Background Reference 2:
Mandinova, A., et al. J. Clin. Invest. 119(10):3127-3137(2009)
Background Reference 3:
Pignata, C., et al. Adv. Exp. Med. Biol. 665, 195-206 (2009) :
Background Reference 4:
Weiner, L., et al. Cell 130(5):932-942(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-203

List Size:
400 ul

List Price:

$495.00

Datasheet
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