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Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.
Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibody at 4 μg/mL.
Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.
Trial Size

FOXO1 Antibody

CATALOG NUMBER: 5811

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1.
The immunogen is located within the first 50 amino acids of FOXO1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
FOXO1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXO1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXO1
Additional Names:
FOXO1 Antibody: FKH1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A
Protein Accession Number:
NP_002006
Protein Gi Number:
9257222
Ncbi Gene Id Number:
2308
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXO1 Antibody: FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
Background Reference 1:
Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
Background Reference 2:
Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
Background Reference 3:
Brunet A, Bonni A, Zigmond MJ, et al. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell1999; 96:857-68.
Background Reference 4:
Linardic CM. PAX3-FOXO1 fusion gene in rhabdomyosarcoma. Cancer Lett.2008; 270:10-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5811

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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