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45-054 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.
45-054 (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-054 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-054 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (A) and without (B) blocking with the immunising peptide. Detected by chemiluminescence.

FOXO3A Antibody

CATALOG NUMBER: 45-054

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-GAKQASSQSWVPG
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Heart lysates (calculated MW of 71.3kDa according to NP_001446.1). Recommended concentration: 0.1-1 ug/ml. Primary incubation was 1 hour.
Specificity:
Both variants represent identical protein (NP_001446.1 and NP_963853.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXO3
Additional Names:
FOXO3, FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog, FOXO2, forkhead homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1, forkhead box O3
Protein Accession Number:
NP_001446.1
Protein Gi Number:
4503739
Ncbi Gene Id Number:
2309
Background Reference 1:
Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Fusion with MLL in leukemia Cell. 1999 Mar 19;96(6):857-68.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-054

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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