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Western blot analysis of FOXO4 in A-20 cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of FOXO4 in HeLa cells with FOXO4 antibody at 10 μg/mL.
Western blot analysis of FOXO4 in A-20 cell lysate with FOXO4 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

FOXO4 Antibody

CATALOG NUMBER: 5817

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
FOXO4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO4.
The immunogen is located within amino acids 50 - 100 of FOXO4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
FOXO4 antibody can be used for detection of FOXO4 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
FOXO4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXO4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXO4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FOXO4
Additional Names:
FOXO4 Antibody: AFX, AFX1, MLLT7, AFX, Forkhead box protein O4, Fork head domain transcription factor AFX1
Protein Accession Number:
NP_005929
Protein Gi Number:
103472003
Ncbi Gene Id Number:
4303
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXO4 Antibody: FOXO4 is a ubiquitously expressed protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In the absence of PI3K/AKT activation, FOXO4 localizes in the nucleus where it functions as a transcription factor. FOXO4 can also be phosphorylated by JNK following induction of reactive oxygen species (ROS), resulting in transcriptional activation and the induction of a negative feedback mechanism to counteract the ROS. It is through this mechanism that FOXO4 is thought to sensitize cancer cells to doxorubicin-mediated toxicity.
Background Reference 1:
Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
Background Reference 2:
Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
Background Reference 3:
Manning BD and Cantley LC. AKT/PKB signaling: navigating downstream. Cell2007; 129:1261-74.
Background Reference 4:
Essers MA, Weijzen S, de Vries-Smits AM, et al. FOXO transcription factor activation by oxidative stress mediated by the small GTPase Ral and JNK. EMBO J.2004; 23:4802-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5817

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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