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45-625 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-625 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-625 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-625 (0.3ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FOXP2 Antibody

CATALOG NUMBER: 45-625

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cat, Dog, Pig, Cow, Zebrafish
Immunogen:
The immunogen for this antibody is: C-DEVEYQKRRSQKIT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 85kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates and in Rat Brain lysates (calculated MW of 82.6kDa according to NP_683696.1). Recommended concentration: 0.3-1ug/ml.An additional band of unknown identity was also consistently observed at 28-30kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Prostate and Placenta. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize isoform I (NP_055306.1), isoform II (NP_683696.2), isoform IV (NP_683698.2) and isoform V (NP_001166237.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FOXP2
Additional Names:
FOXP2, forkhead box P2 , CAGH44, DKFZp686H1726, SPCH1, TNRC10 , CAG repeat protein 44, forkhead/winged-helix transcription factor, speech and language disorder 1, trinucleotide repeat containing 10
Protein Accession Number:
NP_055306.1,NP_683696.2,NP_683697.2,NP_683698.2,NP_001166237.1
Protein Gi Number:
17017963
Ncbi Gene Id Number:
93986
Background Reference 1:
Lai CS, Fisher SE, Hurst JA, Vargha-Khadem F, Monaco AP. A forkhead-domain gene is mutated in a severe speech and language disorder. Nature 2001 Oct 4;413(6855):519-23

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-625

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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