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Western blot analysis of extracts of various cell lines, using FOXP3 antibody (23-358).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human tonsil using FOXP3 antibody (23-358) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using FOXP3 antibody (23-358) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using FOXP3 antibody (23-358) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using FOXP3 antibody (23-358).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

FOXP3 Antibody

CATALOG NUMBER: 23-358

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human FOXP3
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
A-375
Positive Control 3:
Mouse brain
Positive Control 4:
Rat brain
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FOXP3
Additional Names:
FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin
Ncbi Gene Id Number:
50943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-358

List Size:
100 uL

List Price:

$540.00

Out of stock

Datasheet
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