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In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4ug/ml.
45-626 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
In paraffin embedded Mouse Spleen shows patches of stained cells in the Germinal Centre. Recommended concentration, 2-4ug/ml.

FOXP3 Antibody

CATALOG NUMBER: 45-626

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IHC
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: KRSQRPNKCSNP
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in some patches of thymocytes. Recommended concentration: 2-4ug/ml.Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.
Specificity:
Note - anti-mouse companion product of EB05294
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Foxp3
Additional Names:
Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy, RP23-54C14.1, OTTMUSP00000017952, OTTMUSP00000017961, OTTMUSP00000017962
Protein Accession Number:
NP_473380.1
Protein Gi Number:
16905075
Ncbi Gene Id Number:
20371(mouse);317382(rat);
Background Reference 1:
Brunkow, M.E., Jeffery, E.W., Hjerrild, K.A., Paeper, B., Clark, L.B., Yasayko, S.A., Wilkinson, J.E., Galas, D., Ziegler, S.F. and Ramsdell, F Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 27 (1), 68-73 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-626

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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