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Western blot analysis of lysates from Jurkat cells, using FOXP3 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg FOXP3 antibody. Western blot was performed from the immunoprecipitate using FOXP3 antibody at a dilution of 1:1000.
Western blot analysis of lysates from Jurkat cells, using FOXP3 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

FOXP3 Rabbit Recombinant Antibody (84-050)

CATALOG NUMBER: 84-050

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:1000 - 1:4000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Jurkat
Predicted Molecular Weight:
47kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FOXP3
Additional Names:
JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3
Protein Accession Number:
Q9BZS1
Ncbi Gene Id Number:
50943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-050

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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