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Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of FPGS Antibody in K562, A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma with FPGS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells using FPGS Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.

FPGS Antibody

CATALOG NUMBER: 62-566

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FPGS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the Central region of human FPGS.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FPGS
Additional Names:
Folylpolyglutamate synthase, mitochondrial, Folylpoly-gamma-glutamate synthetase, FPGS, Tetrahydrofolylpolyglutamate synthase, Tetrahydrofolate synthase, FPGS
Protein Accession Number:
Q05932
Protein Gi Number:
1706884
Ncbi Gene Id Number:
2356
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FPGS is the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives.
Background Reference 1:
Sharma,S., et.al., Pharmacogenet. Genomics 18 (12), 1041-1049 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-566

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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