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Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of FRMPD1 in K562 cells with FRMPD1 antibody at 20 μg/mL.
Western blot analysis of FRMPD1 in K562 cell lysate with FRMPD1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

FRMPD1 Antibody

CATALOG NUMBER: 5453

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
FRMPD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1.
The immunogen is located within amino acids 1330 - 1380 of FRMPD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
FRMPD1 antibody can be used for detection of FRMPD1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
FRMPD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FRMPD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FRMPD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FRMPD1
Additional Names:
FRMPD1 Antibody: FRMD2, FRMD2, KIAA0967, FERM and PDZ domain-containing protein 1, FERM domain-containing protein 2
Protein Accession Number:
NP_055722
Protein Gi Number:
239582741
Ncbi Gene Id Number:
22844
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FRMPD1 Antibody: The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.
Background Reference 1:
An N, Blumer JB, Bernard ML. The PDZ and Band 4.1 containing protein Frmpd1 regulates the subcellular location of activator of G-protein signaling 3 and its interaction with G-proteins. J. Biol. Chem.2008; 283:24718-28.
Background Reference 2:
Stenzel N, Fetzer CP, Heumann R, et al. PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. J. Cell Sci.2009; 122:3374-84.
Background Reference 3:
Lee HW, Choi J, Shin H, et al. Preso, a novel PSD-95-interacting FERM and PDZ domain protein that regulates dendritic spine morphogenesis. J. Neurosci.2008; 28:14546-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5453

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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