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Western blot analysis of lysate from human skeletal muscle tissue lysate, using PFKM Antibody S137 at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of lysate from human skeletal muscle tissue lysate, using PFKM Antibody S137 at 1:1000 at each lane.

Fructose 6 Phosphate Kinase Antibody

CATALOG NUMBER: 63-418

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This Fructose 6 Phosphate Kinase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the N-terminal region of human Fructose 6 Phosphate Kinase.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
85 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PFKM
Additional Names:
ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255, HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255, HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255, HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255, HAMAP-Rule:MF_03184}, PFKM, PFKX
Protein Accession Number:
P08237
Protein Gi Number:
125126
Ncbi Gene Id Number:
5213
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Background Reference 1:
Howard, T.D., et al., Genomics 34(1):122-127 (1996).
Background Reference 2:
Vasconcelos, O., et al., Proc. Natl. Acad. Sci. U.S.A. 92(22):10322-10326 (1995).
Background Reference 3:
Raben, N., et al., J. Biol. Chem. 268(7):4963-4967 (1993).
Background Reference 4:
Yamasaki, T., et al., Gene 104(2):277-282 (1991).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-418

List Size:
400 ul

List Price:

$495.00

Datasheet
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