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45-057 (0.1ug/ml) staining of Human (A), (0.03ug/ml) of Mouse (B) and (0.3ug/ml) of Pig (C) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-057 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-057 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic and plasma membrane stain
45-057 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane staining. The nuclear stain is DA
45-057 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
45-057 (0.1ug/ml) staining of Human (A), (0.03ug/ml) of Mouse (B) and (0.3ug/ml) of Pig (C) Liver lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FTCD Antibody

CATALOG NUMBER: 45-057

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow
Immunogen:
The immunogen for this antibody is: CLREQGRGKDQPGRL
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Liver lysates and approx. 55-60kDa in Mouse and Pig Liver lysates, and also in preliminary testing of Rat Liver, Human Kidney and U2OS lysates (calculated MW of 58.9kDa according to Human NP_006648.1, Mouse NP_543121.1 and Pig NP_999440.1). Recommended concentration: 0.03-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Liver shows distinct staining of membranes and cytoplasm in a selection of hepatocytes. Recommended concentration: 3-5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm, membranes and plasma membranes of HeLa and in the plasma membranes of HepG2 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Specificity:
Variants (NP_006648.1; NP_996848.1) encode the same protein.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FTCD
Additional Names:
FTCD, LCHC1, formiminotransferase cyclodeaminase, formimidoyltransferase cyclodeaminase, LC1 autoantigen
Protein Accession Number:
NP_006648.1,NP_996848.1,NP_001307341.1
Protein Gi Number:
11140815
Ncbi Gene Id Number:
10841
Background Reference 1:
Bloom GS, Brashear TA. A novel 58- kDa protein associates with the Golgi apparatus and microtubules. J Biol Chem. 1989 Sep 25;264(27):16083-92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-057

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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