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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of FTH1 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with FTH1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of HepG2 cells using FTH1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.

FTH1 Antibody

CATALOG NUMBER: 62-521

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FTH1
Additional Names:
Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein, Ferritin heavy chain, N-terminally processed, FTH1, FTH, FTHL6
Protein Accession Number:
P02794
Protein Gi Number:
120516
Ncbi Gene Id Number:
2495
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Background Reference 1:
Almeida,R.S., et.al., PLoS Pathog.(11), E1000217 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-521

List Size:
400 ul

List Price:

$495.00

Datasheet
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