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IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Western blot testing of human 1) A431, 2) HeLa, 3) liver and 4) testis lysate with Ferritin Light Chain antibody (clone FTL/1386).
SDS-PAGE Analysis of Purified, BSA-Free Ferritin Light Chain Antibody (clone FTL/1386). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

FTL1 Antibody [FTL/1386]

CATALOG NUMBER: 33-711

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Western blot: 0.1-0.2 ug/ml
Flow Cytometry: 0.1-0.2ug/10^6 cells
IF: 0.1-0.2 ug/ml
IHC (FFPE): 0.1-0.2 ug/ml for 30 min at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG2b
Physical State:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
FTL
Additional Names:
FTL, MGC71996, LFTD, NBIA3
Ncbi Gene Id Number:
2512
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-711

List Size:
100 ug

List Price:

$626.00

Datasheet
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