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Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of FTO in mouse brain tissue with FTO antibody at 2.5 μg/mL.
Immunofluorescence of FTO in Mouse Brain cells with FTO antibody at 20 μg/mL.
Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 μg/mL.
Green Product
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FTO Antibody

CATALOG NUMBER: 5137

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
FTO antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FTO.
The immunogen is located within the first 50 amino acids of FTO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FTO antibody can be used for detection of FTO by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1317 - Human Uterus Tissue Lysate
Purification:
FTO Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
FTO Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FTO antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FTO
Additional Names:
FTO Antibody: ALKBH9, KIAA1752, Alpha-ketoglutarate-dependent dioxygenase FTO, Fat mass and obesity-associated protein
Protein Accession Number:
Q9C0B1
Protein Gi Number:
148841515
Ncbi Gene Id Number:
79068
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FTO Antibody: Rising obesity rates are rapidly becoming a growing health concern in the developing world. The fat mass and obesity associated gene (FTO) is the first gene discovered to contribute to common forms of human obesity. FTO is a member of the non-heme dioxygenase superfamily, encoding a 2-oxoglutarate-dependent nucleic acid demethylase whose mRNA is widely expressed, especially in neurons of feeding-related nuclei of the brain. FTO mRNA in the arcuate nucleus in mice is up-regulated by feeding and down-regulated during fasting, although the opposite pattern has been observed in rats. At least four isoforms of FTO are known to exist.
Background Reference 1:
Scuteri A, Sanna S, Chen W-M, et al. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits. PloS Genet.2007; 3:e115.
Background Reference 2:
Gerken T, Girard CA, Tung YCL, et al. The obesity-associated FTO gene encodes a 2-oxyglutarate-dependent nucleic acid demethylase. Science2007; 318:1469-72.
Background Reference 3:
Fredriksson R, Hagglund M, Olszewski PK, et al. The obesity gene, FTO, is of ancient origin, upregulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain. Endocrinology2008; 149:2062-71.
Background Reference 4:
Stratigopoulous G, Padilla S, Leduc CA, et al. Regulation of FTO/FTM gene expression in mice and humans. Am. J. Physiol. Regul. Integr. Comp. Physiol.2008; 294:R1185-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5137

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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