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45-641 (0.01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing Fumarase and probed with 45-641 (mock transfection in first lane).
45-641 (1.5ug/ml) as the reporter antibody.
45-641 (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-641 (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-641 (0.01ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Fumarase Antibody

CATALOG NUMBER: 45-641

Clonality:
Polyclonal
Tested Applications:
EIA, ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-HPNDHVNKSQSSND
Conjugate:
Unconjugated
Tested Applications:
EIA, ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 48kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FH
Additional Names:
fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata
Protein Accession Number:
NP_000134.2
Protein Gi Number:
19743875
Ncbi Gene Id Number:
2271
Background Reference 1:
Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP. Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer Cell. 2007 Apr;11(4):311-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-641

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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