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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with 43-351 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-351 (1ug/ml) in Lane B. Primary
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with 43-351 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with 43-351 (1ug/ml) in Lane B. Primary

FURIN / PCSK3 Antibody

CATALOG NUMBER: 43-351

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RTKRDVYQEPTDP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human Furin (myc and DYKDDDDK tagged), a band of approx. 110kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FURIN / PCSK3
Additional Names:
FURIN, furin (paired basic amino acid cleaving enzyme), FUR, PACE, PCSK3, SPC1, FES upstream region, dibasic processing enzyme, furin, furin, membrane associated receptor protein, paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein), proprotein convertase subtilisin/kexin type 3
Protein Accession Number:
NP_002560.1
Protein Gi Number:
120611
Ncbi Gene Id Number:
5045
Background Reference 1:
Jin W, Wang X, Millar JS, Quertermous T, Rothblat GH, Glick JM, Rader DJHepatic proprotein convertases modulate HDL metabolism

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-351

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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