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Western blot analysis of extracts of various cell lines, using FUT4 antibody (16-551) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using FUT4 antibody (16-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using FUT4 antibody (16-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using FUT4 antibody (16-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using FUT4 antibody (16-551) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using FUT4 antibody (16-551) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using FUT4 antibody (16-551) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

FUT4 Antibody

CATALOG NUMBER: 16-551

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human FUT4 (NP_002024.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
Mouse stomach
Positive Control 3:
Rat testis
Positive Control 4:
Rat lung
Predicted Molecular Weight:
Observed: 95kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
FUT4
Additional Names:
Alpha-(1,3)-fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A
Ncbi Gene Id Number:
2526
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-551

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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