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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
FUT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

FUT4 Antibody

CATALOG NUMBER: 56-210

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This FUT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-524 amino acids from the C-terminal region of human FUT4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FUT4
Additional Names:
Alpha-(1,3)-fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A
Protein Accession Number:
P22083
Protein Gi Number:
226694189
Ncbi Gene Id Number:
2526
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).
Background Reference 1:
Yang, X.S., et al. J. Cell. Physiol. 225(2):612-619(2010) Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010) Pruszak, J., et al. Stem Cells 27(12):2928-2940(2009) Ogata, K., et al. Haematologica 94(8):1066-1074(2009) Read, T.A., et al. Cancer Cell 15(2):135-147(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-210

List Size:
400 ul

List Price:

$495.00

Datasheet
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