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FUT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Western blot analysis of lysate from Hela cell line, using FUT8 Antibody at 1:1000 at each lane.
FUT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

FUT8 Antibody

CATALOG NUMBER: 55-648

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Pig, Bovine
Immunogen:
This FUT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-357 amino acids from the Central region of human FUT8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
67 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FUT8
Additional Names:
Alpha-(1,6)-fucosyltransferase, Alpha1-6FucT, Fucosyltransferase 8, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase, GDP-fucose--glycoprotein fucosyltransferase, Glycoprotein 6-alpha-L-fucosyltransferase, FUT8
Protein Accession Number:
Q9BYC5
Protein Gi Number:
20138326
Ncbi Gene Id Number:
2530
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This enzyme belongs to the family of fucosyltransferases.The product of this gene catalyzes the transfer of fucose fromGDP-fucose to N-linked type complex glycopeptides. This enzyme isdistinct from other fucosyltransferases which catalyze alpha1-2,alpha1-3, and alpha1-4 fucose addition. The expression of this genemay contribute to the malignancy of cancer cells and to theirinvasive and metastatic capabilities. Alternatively splicedvariants encoding different isoforms have been identified.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Wang, X., et al. J. Biochem. 145(5):643-651(2009)
Background Reference 3:
Kudo, T., et al. Mol. Cancer 6, 32 (2007) :
Background Reference 4:
Ihara, H., et al. Glycobiology 16(4):333-342(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-648

List Size:
400 ul

List Price:

$495.00

Datasheet
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