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45-058 (1ug/ml) staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-058 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-058 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
45-058 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
45-058 (1ug/ml) staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

FXR1 Antibody

CATALOG NUMBER: 45-058

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RIEGDNENKLPRED
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FXR1
Additional Names:
FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1
Protein Accession Number:
NP_005078.2,NP_001013456.1,NP_001013457.1
Protein Gi Number:
61835148
Ncbi Gene Id Number:
8087
Background Reference 1:
Coon H, Matsunami N, Stevens J, Miller J, Pingree C, Camp NJ, Thomas A, Krasny L, Lainhart J, Leppert MF, McMahon W. Evidence for linkage on chromosome 3q25-27 in a large autism extended pedigree. Hum Hered. 2005;60(4):220-6. Epub 2006 Jan 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-058

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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